A helium burst biolistic device adapted to penetrate fragile insect tissues

A helium burst biolistic device adapted to penetrate fragile insect tissues

To compensate for the extremely low penetration efficiency of the original PDS/1000-He Bio Rad biolistic device and the deleterious blast effect, design modifications have been made to the launching module. These modifications were evaluated on Bombyx mori embryos and fragile tissues, such as oocytes and imaginal wing disks. The original floppy macrocarrier was replaced by a rigid macrocarrier ...

Targeted biolistics for improved transformation of Impatiens balsamina.

A transgenesis programme has been developed for Impatiens balsamina that will allow elucidation of the roles played by individual genes in the flower reversion phenomenon shown by this model species. The lack of explants exhibiting adventitious shooting in I. balsamina hinders Agrobacterium-based transformation, but the multiple shoots that arise from cotyledonary nodes present a suitable targe...

Low-voltage electric-discharge biolistic device.

dilution is limited primarily in its sensitivity. However, this is of concern only if very small numbers of organisms are suspected in a particular specimen, because the lower limits of detection for a 10-μL aliquot of undiluted material would be approximately 100 cfu/mL. There exists the possibility of crossing of paths as the plates are tilted. We experienced this problem only: (i) on media t...

Damage studies on irradiated tungsten by helium and argon ions in a plasma focus device

Damage of tungsten due to helium and argon ions of a PF device was studied. Tungsten samples were irradiated by 20 shots of the plasma focus device with argon and helium as working gases, separately. The tungsten surface was analyzed by SEM, before and after irradiation. SEM revealed dense blisters with diameters of a few hundred nanometers, on the samples which were irradiated by helium ions, ...

Differential stains of insect tissues.